5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9] - 50ul
Background
5-hydroxymethylcytosine (5-hmC), as a sixth DNA base with functions in transcription regulation, has been detected to be abundant in human and mouse brain and embryonic stem (ES) cells. In mammals, it can be generated by oxidation of 5-methylcytosine (5-mC), a reaction mediated by the ten-eleven translocation (TET) family of 5-mC hydroxylases. 5-hmC was demonstrated to play an important and different role than 5-mC in the regulation of DNA methylation, chromatin remodeling, and gene expression.
Concentration
1 mg/ml
Description
Mouse monoclonal antibody to 5-Hydroxymethylcytosine (5-hmC), clone HMC/4D9
Specificity
Modified base 5-hydroxymethylcytosine (5-hmC), a broad range of species
Isotype
IgG1
Formulation
10 mM phosphate buffer, 150 mM NaCl, pH 7.4
Storage
4°C. For long-term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance. Stable for 6 months from the date of shipment.
Purity
Protein A purified
Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap
Alternative Names
5-hydroxymethylcytidine, anti-5-hydroxymethylcytidine, anti-5-hydroxymethylcytosine, anti-5hmC, anti-5-hmC, anti-5-hmeC, anti-5hmeC, 5hmC, 5hmeC, 5-hmeC
Application
Immunofluorescence: 1:200 – 1: 500, Immunohistochemistry: 1:200 – 1: 500, ELISA: 1:1000 –1:2000, Dot Blot: 1:2000, hMeDIP: 0.5-1 µg/106 Cells