Host: E. coli
Purity: >75%
Form: Liquid
Buffer: 50mM sodium phosphate, pH7.5, 300mM NaCl, 1mM DTT, 10% glycerol.
Concentration: 2.0 pmol/ul (lot specific)
Species: Alicyclobacillus acidiphilus
Scientific Background: CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria. This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development. AapCas12b belongs to the type V CRISPR effector, CRISPR-Cas12b/C2c1, and due to its thermostability, it can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation.
Preparation and Storage: Ships on dry ice.
Aliquot into smaller quantities after centrifugation and store at 70°C. Avoid repeated handling and multiple freeze/thaw cycles.