Product Overview:
The genotype of AGL1 (pSoup) Chemically Competent Cell is C58 RecA (rif R/carb R) Ti pTiBo542DT-DNA (Strep R) Succinamopine(pSoup-tet R). Background of AGL1 strain is C58, RecA-type. It contains rifampicin resistant gene (rif) and the carbenicillin resistance gene (carb) as screening label. AGL1 carries amber basic Ti plasmid pAGL1 (pTiBo542DT-DNA) to facilitate transformation. Ti plasmid pAGL1 (pTiBo542DT-DNA) contains vir gene, which is essential for insertion of T-DNA into plant genome. Ti plasmid pAGL1 (pTiBo542DT-DNA) is disabled to transfer its own T-DNA but enabled to transfer foreign binary vector T-DNA. pAGL1 (pTiBo542DT-DNA) Ti plasmid contains streptomycin resistance gene. The pSoup plasmid helps the pGreen, 62SK, pGs2 series plasmids to replicate in Agrobacterium.
Applications: AGL1 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Notes:
1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.
Transformation efficiency:
Transformation efficiency of EHA105 (pSoup) Chemically Competent Cell using pGs2 plasmid (kanamycin resistance) with 50 μg/mL kan is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.
Storage: Store at - 80 °C for 12 months.